The CalEPR Center at UC Davis includes five EPR instruments with frequencies ranging from 9 to 130 GHz. Advanced EPR capabilities include ESEEM, HYSCORE, and CW and pulsed ENDOR. All instrumentation has been recently moved to a newly renovated contiguous lab space within the basement of the UC Davis Chemistry Building.
To schedule time on the instrumentation, please go to the CALEPR scheduler website .
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Bruker ElexSys 580 (9 and 35 GHz) |
This new state-of-the-art instrument provides a broad spectrum of CW and pulsed capabilities at X band (9 GHz) and Q band (35 GHz) frequencies. It is capable of CW ENDOR at X band, and pulsed ENDOR at X and Q bands. (This is one of the very first Bruker pulsed Q band instruments
to be installed in the U.S.A.).
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Routine X band (9 GHz) continuous wave (CW) EPR is performed on a Bruker ECS 106 instrument, equipped with an Oxford liquid He cryostat for low temperature work. The instrument is show with the Bruker dual mode cavity, which allows for parallel as well as standard perpendicular EPR experiments. |
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This "lab-built" pulsed EPR instrument works over a broad frequency range (from 8 to 18 GHz) (Sturgeon and Britt (1992) Rev. Sci. Instrum. 63:2187-2192). It has been our workhorse instrument for many years for ESEEM and pulsed ENDOR experiments.
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This "lab-built" Ka-band (31 GHz) instrument performs pulsed EPR using a 100 W travelling wave tube amplifier for high microwave power in this intermediate frequency range. To date it has mostly been used for ESEEM experiments. We are working to implement pulsed ENDOR capabilities.
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This D-band (130 GHz) pulsed EPR instrument is based on a bridge and probe provided by HF EPR Instruments. We recently received and installed a 0-8 Tesla magnet which employs a cryogen free refrigeration system. This magnet will allow us to access the entire range of g values at this microwave frequency. The second photograph shows the tiny cavity and sample tubes used at this high frequency. This instrument is used for high frequency pulsed EPR and ENDOR studies.
The cavity for the D Band instrument (below) is quite small; total sample volume is approximately 0.5 µl . The small volume makes it ideal for mass limited samples and single crystal studies of proteins The cavity is a TE-011 mode cylindrical cavity with slits that permit RF penetration for ENDOR studies.
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